![]() The motion controller receives the information from the stepper motor, and the signals of the force sensor generated by the loading are processed by the amplifier. The connection wires from the bioreactor are plugged over an electronic box (4) that contains a motion controller, an amplifier of the force sensor and the interpolator for the digital gauge. The bioreactor (1) connected to a power supply (3) is placed inside a CO 2 incubator to allow conditions for cell cultivation. The system of the bioreactor is designed as standalone unit. An alginate scaffold C) and the elastic ring D) are shown from above and a skew side view in detail. The mesh aims to prevent the scaffold from moving up during the lift maneuvers. B) The schematic drawing shows the cross-section of the parts of an assembled scaffold holder without the upper anchored ring. The cartridge is made of sterilizable materials. The scaffold holder is placed as an independent movable unit within the cylindrical container (1) and it is covered up by a cap (7) containing the piston and spring. The mesh is kept in place by a ring anchored to the construction using screws (6). A scaffold (4) is placed over them, which is held in place by the elastic ring (3) and a mesh above it (5). The scaffold holder (2) contains the reservoir in the middle where the cells are placed. Dimensions of the bioreactor: 120 x 150 x 400 mm (width, depth, height), approximately.Ī) The sketch shows parts of a disassembled cartridge. ![]() A force sensor is located underneath the membrane, which is mandatory for reliable measurements of the induced forces. A non-permeable membrane forms the bottom of the cartridge, isolating the cell cultivation system to protect it from contamination. The piston compresses simultaneously the elastic ring and the scaffold (not shown) located over the cell reservoir. B) The technical sketch depicts the inner structures of the bioreactor. ![]() The cartridge cap comprises the embedded piston and the vents for gas exchange covered by 0.22 μm filters to protect from the external environment. It can be disassembled or assembled and hold to the bioreactor chassis by fixation screws. The cartridge is the housing for cell cultivation and mechanical application of the strain in the scaffold. Parallel to the stepper motor, a digital gauge measures independently the vertical displacements of the piston. A stepper motor drives the piston downwards and a spring (not visible) covered by a white bellows moves it upward. A) An authentic picture of the compression bioreactor is shown. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |